Characterization & modelling of single-use chromatographic clarification to replace endonuclease treatment in downstream processing of recombinant adeno-associated viral vectors
Time: 9:00 am
day: Conference Day 1 - AM
Details:
- Current manufacturing processes for recombinant adeno-associated viral vectors (rAAV), endonuclease treatment is used prior to depth filtration to break down large amounts of chromatin post-cellular lysis of the HEK293 host cells.
- Single-use chromatographic clarification uses a next-generation synthetic fibrous anionexchange clarification media to simultaneously remove (i) solids through filtration, (ii) soluble negatively charged impurities, and (iii) sterile filter cell harvest material through a terminal 0.2 um membrane.
- These novel filters offer a unique benefit for rAAV production as removal of chromatin from the bioreactor harvest material by filtration, presents an opportunity to eliminate expensive endonuclease treatment
